Part:BBa_K1383000:Experience

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Applications of BBa_K1383000

This part was amplified via PCR and inserted into a vector with a constitutive promoter. By expressing this mCherry gene with the native RBS in Methanococcus maripaludis we detected fluorescence with the use of a plate reader.

New Application of the Part UGA-Georgia 2015 iGEM

After development of this part along with BBa_K1383001 and BBa_K1383002, this part has been applied as a standard for our point mutation library. Rather than only being an expression tool for our developing library, it has become the sequence by which all our mutations are standardized against. This can be seen in Table 1 where the fluorescence values are all percentages of relative native expression.

UGA-Georgia 2015 Figure 6: This graph depicts point mutation versus percent of native sequence fluorescence. The native's full sequence is depicted along the x-axis at 100% fluorescence. Not all values shown here have been made into parts, the only part shown is the G to T point mutation at the 4th base site (BBa_K1635000).

UGA-Georgia 2015 Table 1: This table shows the percent of native expression for 8 different mutations of the native sequence as well as the native sequence. This table includes both pre-existing parts and what will become future parts.

User Reviews

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